Muscle foods, notably red meat, poultry meat, and fish are the first choice of animal source food with adequate protein for human and thus unstable. The present study was undertaken to examine the preservative and antimicrobial potentials of Phoenix dactylifera L. gel and its ethanolic extract against spoilage microorganisms from muscle foods sold in Akure, Southwest Nigeria. Muscle foods namely: beef (Bos taurus), chicken (Gallus gallus domesticus), turkey (Meleagris gallopavo), pork (Sus scrofa domesticus), chevon (Capra aegagrus hircus), mackerel (Scomber scombrus), horse mackerel (Trachurus trachurus), herrings (Clupea pallasii), blue whiting (Merluccius merluccius) and croaker (Micropogonias undulatus) were collected from different locations in Akure, Nigeria. Pathogenic food spoilage microorganisms, Clinical isolates and American typed cultures were obtained from the Microbiological Laboratory Federal University of Technology Akure, Ondo State Nigeria, Obafemi Awolowo University Teaching Hospital Ile-ife, Osun State Nigeria and Medical Microbiology Laboratory University College Hospital (UCH) Ibadan, Oyo State Nigeria. The preservative and antimicrobial potentials of Phoenix dactylifera L. gel and its ethanolic extract were examined against the microorganisms. Proximate, phytochemical and bioactive components of Phoenix dactylifera L. gel were as well evaluated. Data were analyzed using SPSS software version 17.0. All muscle foods were well preserved for three (3) days, then spoilage. Phoenix dactylifera L. gel and its ethanolic extract displayed a strong antimicrobial activity on all tested organisms.